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automated hybridization station hs4800  (Tecan Systems)


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    Structured Review

    Tecan Systems automated hybridization station hs4800
    Automated Hybridization Station Hs4800, supplied by Tecan Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hybridization+station/hs4800+hybridization+station/pm40664182-92-7-10
    Average 90 stars, based on 1 article reviews
    automated hybridization station hs4800 - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Labeling:

    Article Title: Microenvironment dependent gene expression signatures in reprogrammed human colon normal and cancer cell lines
    Article Snippet: Briefly, 0,5 μg of total RNA was used for cDNA synthesis and amplification using the MessageTMAmp aRNA kit (ThermoFisher Scientific). .. Then 825 ng of cRNA labeled with Cy3/Cy5 dyes using the Arcturus® TURBO labelingTM CyTM3/CyTM5 Kit (Applied Biosystems, Netherlands) were hybridized to Human 4x44k Oligonucleotide Microarrays (Agilent Technologies, USA) using the HS 400 hybridization station (Tecan, Switzerland). .. Microarray slides were scanned using a LS Reloaded scanner (Tecan, Switzerland) for microarray image analysis, and the data generated were further analyzed using ImaGene ver.

    Article Title: The rectal cancer microRNAome –microRNA expression in rectal cancer and matched normal mucosa
    Article Snippet: .. The labeled RNA was hybridized to miRCURY arrays for 16 hours at 65°C in a Tecan HS4800 hybridization station. .. After washing and drying, the microarrays were scanned in an Agilent G2565BA Microarray Scanner System (Agilent Technologies, Santa Clara, CA) and the images were quantified using ImaGene® v. 8.0 (BioDiscovery, El Segundo, CA).

    Hybridization:

    Article Title: Microenvironment dependent gene expression signatures in reprogrammed human colon normal and cancer cell lines
    Article Snippet: Briefly, 0,5 μg of total RNA was used for cDNA synthesis and amplification using the MessageTMAmp aRNA kit (ThermoFisher Scientific). .. Then 825 ng of cRNA labeled with Cy3/Cy5 dyes using the Arcturus® TURBO labelingTM CyTM3/CyTM5 Kit (Applied Biosystems, Netherlands) were hybridized to Human 4x44k Oligonucleotide Microarrays (Agilent Technologies, USA) using the HS 400 hybridization station (Tecan, Switzerland). .. Microarray slides were scanned using a LS Reloaded scanner (Tecan, Switzerland) for microarray image analysis, and the data generated were further analyzed using ImaGene ver.

    Article Title: The rectal cancer microRNAome –microRNA expression in rectal cancer and matched normal mucosa
    Article Snippet: .. The labeled RNA was hybridized to miRCURY arrays for 16 hours at 65°C in a Tecan HS4800 hybridization station. .. After washing and drying, the microarrays were scanned in an Agilent G2565BA Microarray Scanner System (Agilent Technologies, Santa Clara, CA) and the images were quantified using ImaGene® v. 8.0 (BioDiscovery, El Segundo, CA).

    Article Title: IL-1β-induces NF-κB and upregulates microRNA-372 to inhibit spinal cord injury recovery.
    Article Snippet: Total RNA including miR was isolated from cultured cells at the indicated conditions using Trizol (Invitrogen, Carlsbad, CA) and was labeled as the first-strand biotin-cDNA. .. The hybridization steps were performed on a hybridization station (Tecan) followed by an indirect detection of streptavidin-Alexa Fluor 647 conjugate (Invitrogen). .. The image analysis of microarray was carried out using GenePix Pro (Molecular Devices, Sunnyvale, CA).

    Article Title: IL-11 promotes the treatment efficacy of hematopoietic stem cell transplant therapy in aplastic anemia model mice through a NF-κB/microRNA-204/thrombopoietin regulatory axis
    Article Snippet: Total RNA, including miRNA, was isolated from cultured cells at the indicated conditions using TRIzol (Invitrogen, Carlsbad, CA, USA) and was labeled as first-strand biotin-cDNA. .. The hybridization steps were performed on a hybridization station (Tecan) followed by an indirect detection of streptavidin-Alexa647 conjugate (Invitrogen). .. The microarray image analysis was carried out using GenePix Pro software (Molecular Devices, Sunnyvale, CA, USA).

    Article Title: IL-1β-induces NF-κB and upregulates microRNA-372 to inhibit spinal cord injury recovery
    Article Snippet: Total RNA including miR was isolated from cultured cells at the indicated conditions using Trizol (Invitrogen, Carlsbad, CA) and was labeled as the first-strand biotin-cDNA. .. The hybridization steps were performed on a hybridization station (Tecan) followed by an indirect detection of streptavidin-Alexa Fluor 647 conjugate (Invitrogen). .. The image analysis of microarray was carried out using GenePix Pro (Molecular Devices, Sunnyvale, CA).

    Article Title: Ferulic acid promotes osteogenesis of bone marrow-derived mesenchymal stem cells by inhibiting microRNA-340 to induce β-catenin expression through hypoxia.
    Article Snippet: Osteogenic differentiation is regulated through multiple signaling networks that may include responses to hypoxia.. Antioxidant ferulic acid (FA) can promote hypoxia signaling by inducing hypoxic-induced factor (HIF).. However, whether FA could affect osteogenesis has not been explored.

    Article Title: Soil-Borne Microbial Functional Structure across Different Land Uses
    Article Snippet: .. The amplified DNAs were labelled with a Cy5 fluorescent dye, purified, and then hybridized to the GeoChip functional array (FGA II) [ ] in a Tecan hybridization station (Durham, NC) at 42°C for 10 hours. ..

    Article Title: NF-κB-Induced MicroRNA-211 Inhibits Interleukin-10 in Macrophages of Rats with Lipopolysaccharide-Induced Acute Respiratory Distress Syndrome.
    Article Snippet: Reverse transcription was performed with the SuperScript II Reverse Transcriptase (ThermoFisher) and biotin labeling was performed with the BioArray High Yield RNA Transcript Labeling kit (Enzo). .. The hybridization steps were performed on the hybridization station (Tecan) followed by an indirect detection of streptavidinAlexa647 conjugate (ThermoFisher Scientific, S21374, USA). .. The image was analyzed by GenePix pro (Molecular Devices, Sunnyvale, CA, USA).

    Amplification:

    Article Title: Soil-Borne Microbial Functional Structure across Different Land Uses
    Article Snippet: .. The amplified DNAs were labelled with a Cy5 fluorescent dye, purified, and then hybridized to the GeoChip functional array (FGA II) [ ] in a Tecan hybridization station (Durham, NC) at 42°C for 10 hours. ..

    Purification:

    Article Title: Soil-Borne Microbial Functional Structure across Different Land Uses
    Article Snippet: .. The amplified DNAs were labelled with a Cy5 fluorescent dye, purified, and then hybridized to the GeoChip functional array (FGA II) [ ] in a Tecan hybridization station (Durham, NC) at 42°C for 10 hours. ..

    Functional Assay:

    Article Title: Soil-Borne Microbial Functional Structure across Different Land Uses
    Article Snippet: .. The amplified DNAs were labelled with a Cy5 fluorescent dye, purified, and then hybridized to the GeoChip functional array (FGA II) [ ] in a Tecan hybridization station (Durham, NC) at 42°C for 10 hours. ..



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